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Career Opportunities

Research Technologist- Camargo Lab

This position is located within the Camargo Lab at Boston Children’s Hospital and calls for a highly motivated, intelligent, responsible, conscientious, and detail-oriented individual. The successful candidate must be a quick learner who enjoys acquiring new skills, advancing projects independently and collaboratively.

Research Assistant I - Kim Lab

The Stem Cell Program at Boston Children’s Hospital seeks applications from highly motivated candidates for the position of Research Assistant I in the Carla Kim Laboratory, which is focused on the study of lung stem cell biology, regenerative medicine and lung disease research. Candidate will have the opportunity to support multiple research projects and will conduct a variety of techniques such as lung organoid culture, immunohistochemistry, flow cytometry, single cell RNA-seq, and other molecular biology techniques. Further responsibilities may include collecting and processing human patient samples, purchasing and maintenance of laboratory stocks, animal colony management and husbandry, equipment maintenance and maintenance of records and documentation to ensure laboratory’s compliance with institutional and federal guidelines in health, safety and animal regulations.

Postdoctoral Fellowship Position: Stem Cell Program in Carla Kim Lab

The Kim Lab in the Stem Cell Program at Boston Children’s Hospital seeks a highly motivated postdoctoral research fellow, who will conduct research under the mentorship of Dr. Carla Kim, Professor of Genetics at Harvard Medical School, Harvard Stem Cell Institute Faculty Member, and Member of the Stem Cell Program at Boston Children’s Hospital.

 

Learn more about the position 

The broad interest of the Kim Lab is to use stem cell biology to increase our understanding of how mechanisms governing normal lung biology are impacted in lung disease and lung cancer. Our lab utilizes co-culture organoid systems that allow us to define how mouse and human lung epithelial progenitor cells are regulated by cell-cell communication. Multiple projects are aimed at using these systems to shed light on lung cancer, emphysema, COPD, idiopathic pulmonary fibrosis and other lung diseases. The position offers an exciting opportunity to work closely with scientists and medical professionals in the highly collaborative and stimulating environment of the Stem Cell Program at Boston Children’s Hospital located in the vibrant Longwood / Harvard Medical Area.

There are several projects in the Kim lab for postdoctoral research fellows, including but not limited to:

  • Transplantation of lung organoids to repair damaged lungs. The Kim lab is exploring the transplantation of healthy lung organoids as a tool for understanding lung stem cell biology and as a possible means to future cell-based therapy in diseased lungs.  

  • Organoid models of early stage lung cancer. We are using lung organoids that carry tumorigenic mutations to model the early steps of lung adenocarcinoma and devise new strategies to impair the progression of small lesions to full malignant growth.   

Recent Kim Lab publications: 

Progenitor potential of lung epithelial organoid cells in a transplantation model. 
Louie SM, Moye AL, Wong IG, Lu E, Shehaj A, Garcia-de-Alba C, Ararat E, Raby BA, Lu B, Paschini M, Bronson RT, Kim CF. 
Cell Rep. 2022 Apr 12;39(2):110662. doi: m10.1016/j.celrep.2022.110662. 

Organoids Model Transcriptional Hallmarks of Oncogenic KRAS Activation in Lung Epithelial Progenitor Cells. 
Dost AFM, Moye AL, Vedaie M, Tran LM, Fung E, Heinze D, Villacorta-Martin C, Huang J, Hekman R, Kwan JH, Blum BC, Louie SM, Rowbotham SP, Sainz de Aja J, Piper ME, Bhetariya PJ, Bronson RT, Emili A, Mostoslavsky G, Fishbein GA, Wallace WD, Krysan K, Dubinett SM, Yanagawa J, Kotton DN, Kim CF. 
Cell Stem Cell. 2020 Oct 1;27(4):663-678.e8. doi: 10.1016/j.stem.2020.07.022. Epub 2020 Sep 4. 

BRG1 Loss Predisposes Lung Cancers to Replicative Stress and ATR Dependency. 
Gupta M, Concepcion CP, Fahey CG, Keshishian H, Bhutkar A, Brainson CF, Sanchez-Rivera FJ, Pessina P, Kim JY, Simoneau A, Paschini M, Beytagh MC, Stanclift CR, Schenone M, Mani DR, Li C, Oh A, Li F, Hu H, Karatza A, Bronson RT, Shaw AT, Hata AN, Wong KK, Zou L, Carr SA, Jacks T, Kim CF. 
Cancer Res. 2020 Sep 15;80(18):3841-3854. doi: 10.1158/0008-5472.CAN-20-1744. Epub 2020 Jul 20. 

Requirements: 

The candidate should have a Ph.D. or an M.D./Ph.D.  No postdoctoral experience is required. 

How to apply: 

Interested applicants should email their CV, a cover letter indicating research experience, interests and goals, and names/contact information for at least three academic references to the Dr. Carla Kim at Carla.Kim@childrens.harvard.edu

Postdoctoral Fellowship Position: Stem Cell Program in Zon Lab

The Zon Lab in the Stem Cell Program at Boston Children’s Hospital seeks a highly motivated postdoctoral research fellow, who will conduct research under the mentorship of Dr. Leonard Zon, Professor of Pediatrics at Harvard Medical School, HHMI Investigator, Harvard Stem Cell Institute Faculty Member, and Director of the Stem Cell Program at Boston Children’s Hospital.

 

Learn more about the position 

Job Summary

The Zon Lab utilizes the zebrafish model system and cutting-edge technologies to study the role of gene expression in hematopoiesis, cancer, and stem cell development. The lab aspires to develop and improve drug therapies and treatments for patients with blood disorders and melanoma. The position offers an exciting opportunity to work closely with scientists in the highly collaborative and stimulating environment of the Stem Cell Program at Boston Children’s Hospital and in the vibrant Longwood / Harvard Medical Area.   

There are several projects in the Zon lab for postdoctoral research fellows: 

  1. Blood stem cells can easily be studied in the zebrafish.  We have created transgenic reporter fish for stem cells and many tissues types in the environment of the stem cells.  Live imaging can elucidate the trafficking of stem cells into the niche and probe mechanisms involved in stem cell regulation.  A focus of the lab is to study transcriptional fate changes to promote stem cell division, proliferation or migration. 
  2. Cellular barcoding of stem cells can be accomplished by creating fish with different colored stem cells or by using a CRISPR-based barcoding system.  This has allowed us to study clones of blood cells and evaluate what leads to clonal dominance.  We can follow cells that transition from clonal hematopoiesis to MDS to leukemia. 
  3. A melanoma model was created using the zebrafish. Using transgenic reporters, we can study the genetics of cancer initiation and probe the cancer niche.  We can position an adult zebrafish with a tumor on a two photon scope and image continuously for 18 hours, watching T cells and dendritic cells track in locations in the tumor.  We are also using barcoding to look at tumor initiation, resistance and response to the immune system. 

 

Recent Zon Lab publications: 

Resistance to inflammation underlies enhanced fitness in clonal hematopoiesis. 
Avagyan S, Henninger JE, Mannherz WP, Mistry M, Yoon J, Yang S, Weber MC, Moore JL, Zon LI.Science. 2021 Nov 5;374(6568):768-772. doi: 10.1126/science.aba9304. Epub 2021 Nov 4.PMID: 34735227

Cell-specific transcriptional control of mitochondrial metabolism by TIF1γ drives erythropoiesis. 
Rossmann MP, Hoi K, Chan V, Abraham BJ, Yang S, Mullahoo J, Papanastasiou M, Wang Y, Elia I, Perlin JR, Hagedorn EJ, Hetzel S, Weigert R, Vyas S, Nag PP, Sullivan LB, Warren CR, Dorjsuren B, Greig EC, Adatto I, Cowan CA, Schreiber SL, Young RA, Meissner A, Haigis MC, Hekimi S, Carr SA, Zon LI.Science. 2021 May 14;372(6543):716-721. doi: 10.1126/science.aaz2740.PMID: 33986176 Free PMC article. 

RNA helicase DDX21 mediates nucleotide stress responses in neural crest and melanoma cells. 
Santoriello C, Sporrij A, Yang S, Flynn RA, Henriques T, Dorjsuren B, Custo Greig E, McCall W, Stanhope ME, Fazio M, Superdock M, Lichtig A, Adatto I, Abraham BJ, Kalocsay M, Jurynec M, Zhou Y, Adelman K, Calo E, Zon LI.Nat Cell Biol. 2020 Apr;22(4):372-379. doi: 10.1038/s41556-020-0493-0. Epub 2020 Mar 30.PMID: 32231306 Free PMC article. 

Requirements: 

The candidate should have a Ph.D. or an M.D./Ph.D.  No postdoctoral experience is required. 

How to apply: 

Interested applicants should email their CV, a cover letter indicating research experience, interests and goals, and names/contact information for at least three academic references to the Zon Lab Manager, Dr. Anne Ellett at Anne.Ellett@Childrens.harvard.edu 

Research Assistant I -Hematology/Oncology

This position is located within a human stem cell biology laboratory at Boston Children’s Hospital and calls for a highly motivated, intelligent, responsible, conscientious, and detail-oriented individual. The successful candidate must be a quick learner who enjoys acquiring new skills, advancing projects independently and collaboratively.

Research Assistant I - Daley Lab

The Daley Lab, a multinational research group at Boston Children’s Hospital, is seeking a highly motivated, responsible, conscientious, and detail-oriented individual to join our ongoing efforts to uncover the molecular mechanisms that regulate stem cell activation, aging, blood development, and malignant transformation. The successful candidate must be a quick learner who enjoys acquiring new skills and working as a member of a highly collaborative team. In addition to providing technical research support to lab members, responsibilities will also include pluripotent stem cell culture, directed differentiation and mice as a model organism for aging studies.