Research | Overview
The intersection of normal and diseased blood formation
The Rowe laboratory is interested in the intersection of normal and diseased blood formation. We incorporate the concept of heterochrony (timing of developmental events) into understanding the process of normal hematopoiesis over the lifetime of an organism. By defining age-specific hematopoietic states, we aim to unlock new insights into the pathobiology of age-biased blood disease. Our work uses primary human blood cells, pluripotent stem cells, experimental models of stem cell transplantation, and single cell functional and -omics assays.
Rowe RG, Lummertz da Rocha E, Sousa P, Missios P, Morse M, Marion W, Yermalovich A, Barragan J, Mathieu R, Fleming MD, Daley GQ. The developmental state of the hematopoietic niche regulates lineage in MLL-rearranged leukemia. J Exp Med 2019; 216:527-538.
Rowe RG, Daley GQ. Induced pluripotent stem cells in disease modeling and drug discovery. Nat Rev Genetics 2019; 20:377-88.
Wang L*, Rowe RG*, Jaimes A, Yu C, Nam Y, Pearson DS, Zhang J, Xie X, Marion W, Heffron G, Daley GQ, Sliz P. Small molecule inhibitors disrupt let-7 oligouridylation and release the selective blockade of let-7 processing by LIN28B. Cell Rep 2018; 5:3091-3101.
Lummertz da Rocha E, Rowe RG, Lundin V, Malleshaiah M, Jha D, Rambo C, Li H, North T, Collins J, Daley GQ. Reconstruction of complex single-cell trajectories using CellRouter. Nat Communications 2018; 9:892.
Sugimura R, Jha DK, Han A, Soria-Valles C, Lummertz da Rocha E, Lu YF, Goettel JA, Serrao E, Rowe RG, Malleshaiah M, Wong E, Sousa P, Zhu TN, Ditaldi A, Keller G, Engelman AN, Snapper SB, Doulatov S, Daley GQ. Hematopoietic stem and progenitor cell from human pluripotent stem cells. Nature 2017; 25: 432-438.
Rowe RG*, Wang LD*, Coma S, Han A, Mathieu R, Pearson DS, Ross S, Sousa P, Nguyen PT, Rodriguez A, Wagers AJ, Daley GQ. Developmental regulation of myeloerythroid progenitor function by the Lin28b-let-7-Hmga2 axis. J Exp Med 2016; 25: 1497-512.
Rowe RG*, Mandelbaum J*, Zon LI, Daley GQ. Engineering hematopoietic stem cells: lessons from development. Cell Stem Cell 2016; 18: 707-20.
Kim P, Nakano H, Das P, Chen M, Rowe RG, Chou S, Ross S, Sakamoto K, Zon L, Schlaeger T, Orkin S, Nakano A, Daley GQ. Flow-induced protein kinase A/CREB pathway acts via BMP signaling to promote HSC emergence. J Exp Med 2015; 212:633-48
Wang L*, Rao T*, Rowe RG*, Nguyen P, Sullivan J, Pearson D, Doulatov S, Wu L, Lindsley R, Zhu H, DeAngelo D, Daley GQ, Wagers A. The role of Lin28 in myeloid and mast cell differentiation and mast cell malignancy. Leukemia 2015; 29:1320.